Un article médical de 2005

[Aghabie 20 060]

Virol J. 2005; 2: 69.

Published online 2005 Aug 22. doi: 10.1186/1743-422X-2-69

PMCID: PMC1232869

PMID: 16115318

Chloroquine is a potent inhibitor of SARS coronavirus infection and spread

Martin J Vincent,¹ Eric Bergeron,² Suzanne Benjannet,² Bobbie R Erickson,¹ Pierre E Rollin,¹ Thomas G Ksiazek,¹ Nabil G Seidah,² and Stuart T Nichol¹

Background

Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available.

Results

We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. In addition to the well-known functions of chloroquine such as elevations of endosomal pH, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations.

Conclusion

Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds.

Keywords: severe acute respiratory syndrome coronavirus, chloroquine, inhibition, therapy https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1232869/

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Un rhume = coronavirus, cet article date de 2005 et ils savaient que la chloroquine empêche le développement du virus, cette comédie mondiale commence vraiment à me casser les pieds mais la réaction des gens m'énerve encore plus !